QMUL Lipid Mediator Unit

Objective of the Unit: The QMUL Lipid Mediator Unit is a QMUL Core Service that provides the instrumentation and expertise to investigate endogenous protective mechanisms  that may become disregulated in disease with a focus on bioactive lipid mediators (Figure 1). 

Overview of the services: The Unit utilizes state of the art Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS; Figure 2) based techniques to identify and quantify bioactive lipid mediators (LM), their precursors and pathway markers in biological systems. The Unit also utilizes LC-MS-MS based techniques to identify and quantify both known and novel further metabolites for each of the bioactive mediators.  Together these provide an insight into the resolution status of the biological system(s) being investigated (e.g. cell incubations, organs, tissues etc), protective pathways that become dysregulated during disease as well as potential novel therapeutic leads.

                            

Location:

William Harvey Research Institute, 1st floor
Charterhouse Square, London, UK, EC1M 6BQ

Equipment

 

Service and Data Analysis

Sample Preparation

Contact us prior to your experiment to discuss procedures and sample preparations

Record tissue weight, cell counts and/or sample volumes

Snap freeze your samples immediately after collection

Avoid freeze/thawing your samples

Storage controls need to be provided for any samples stored for extensive period of time

Phenol red free media should be used in all in vitro experiments – we cannot process tissues and cell culture samples containing phenol red.

Complete the analysis request form – instructions on how to complete this will be provided once you contact us to discuss your specific interests.

Then co-ordinate with members of the Unit to submit your samples and we will do the rest

 

Contact

Dr. Jesmond DALLI

QMUL Lipid Mediator Unit Director
William Harvey Research Institute
Barts and The London School of Medicine and Dentistry
Queen Mary University of London
Charterhouse Square, London. UK. EC1M 6BQ

Phone: +44 207 882 8263

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Dr. Romain A. COLAS

QMUL Lipid Mediator Unit
William Harvey Research Institute
Barts and The London School of Medicine and Dentistry
Queen Mary University of London
Charterhouse Square, London. UK. EC1M 6BQ

Phone: +44 207 882 5646

email:This email address is being protected from spambots. You need JavaScript enabled to view it.